Journal of Clinical Microbiology  1 Dec 2009  V.47  N.12  p.3805-3813

Jason J. LeBlanc,1* Yan Li,3 Nathalie Bastien,3 Kevin R. Forward,1,2 Ross J. Davidson,1,2 and Todd F. Hatchette1,2

Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada,1 Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada,2 National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada3

Rapid methods for the detection and confirmatory identification of pandemic influenza A virus (also known as pandemic [H1N1] 2009) are of utmost importance. In this study, a conventional reverse transcriptase PCR (RT-PCR) assay for the detection of influenza A virus and the hemagglutinin of swine lineage H1 (swH1) was designed, optimized, and validated. Nucleic acids